期刊
THERIOGENOLOGY
卷 156, 期 -, 页码 116-123出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2020.06.038
关键词
Leonurine; Porcine; Embryo development; Reactive oxygen species; Mitochondrial function
资金
- Modern Agricultural Industry Technology System [CARS-38]
- Science and Technology Project of Jilin Province [SXGJSF2017-6]
- Next-Generation BioGreen 21 Program [PJ01322101]
- Rural Development Administration, Republic of Korea
Leonurine (LEO) is pseudoalkaloid that has been isolated from motherwort. It has been found to have various biological activities, including an antioxidant capacity. This study aimed to confirm whether LEO could be used in porcine in vitro culture (IVC) medium for its antioxidant effect and related molecular mechanisms. The results showed that embryos in IVC medium supplemented with 40 mM LEO had an increased blastocyst formation rate, total cell number, and proliferation capacity and a low apoptosis rate. LEO supplementation decreased reactive oxygen species levels and increased glutathione levels. Moreover, LEO-treated embryos exhibited improved intracellular mitochondrial membrane potential and reduced autophagy. In addition, pluripotency related gene was up-regulated while apoptosis and autophagy related genes were down-regulated with LEO supplementation. These results suggest that LEO has a beneficial effect on pre-implantation embryo development by reducing oxidative stress and enhancing mitochondrial function. (c) 2020 Elsevier Inc. All rights reserved.
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