Preparation and application of peptide molecularly imprinted material based on mesoporous metal-organic framework

In this study, a new molecularly imprinted material, MIP@UiO-66-NH2, was synthesized with glutathione (GSH) as template and mesoporous metal organic framework (UiO-66-NH2) as matrix. The molecularly imprinted polymer was modified on the surface and into the pores of the UiO-66-NH2 by surface molecular imprinting method with thin polymer layer. Based on high specific surface area (1091.93 m(2) g(-1)) and appropriate pore size (35 nm) of the ordered mesoporous UiO-66-NH2, the adsorption capacity for GSH reached 94.43 mg g(-1), and the adsorption equilibrium could be achieved within 30 min. The adsorption isotherm data of MIP@UiO-66-NH2 could be described well by Freundlich model and the kinetic data complied well with pseudo-second-order model. In addition, the MIP@UiO-66-NH2 showed low adsorption capacity to GSH structural analogs (Q(L-cys) = 6.51 mg g(-1)), suggesting great selectivity for GSH recognition. Finally, the MIP@UiO-66-NH2 was successfully applied for selective separation of GSH from BSA, skim milk and egg white tryptic digest.

Automated summary

A new molecularly imprinted material, MIP@UiO-66-NH2, with high adsorption capacity for GSH and great selectivity, was successfully synthesized in this study. The material achieved adsorption equilibrium for GSH within 30 minutes and showed low adsorption capacity for structural analogs.