期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 238, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2020.118437
关键词
Fluorescent probe; Nucleophilic substitution-addition; Bioimaging
类别
资金
- National Natural Science Foundation of China [21775096, 21907062]
- One Hundred People Plan of Shanxi Province
- Shanxi Province 1331 project Key Innovation Team Construction Plan Cultivation Team [2018-CT-1]
- 2018 Xiangyuan County Solid Waste Comprehensive Utilization Science and Technology Project [2018XYSDJS-05]
- Shanxi Province Foundation for Returness [2017-026]
- Shanxi Collaborative Innovation Center of High Value-added Utilization of Coal-related Wastes [2015-10-B3]
- Shanxi Province Annual Science and Technology Activities for Overseas Students Selected Funding Projects (2019)
- Innovative Talents of Higher Education Institutions of Shanxi (2019)
- Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi [2019L0031]
- Key R&D Program of Shanxi Province [201903D421069]
- Natural Science Foundation of Shanxi Province of China [201901D111015]
- Xinzhou Teachers University Fund
- Scientific Instrument Center of Shanxi University [201512]
In view of the importance of hydrogen sulfide (H2S) in the organism, a fast, noninvasive method for the detection of H2S in situ is needed. Fluorescent probes based on disulfide-bond nucleophilic substitution-addition can selectively detect H2S in vivo, which is very popular because it allows quick response for H2S, thus it will be a useful tool for monitoring H2S in the vivo. We developed a dicyanoisopentanone-based H2S fluorescent probe (EW-H) that used a disulfide group as a self-destructive linker reaction site. Under the nucleophilic substitution of H2S, the disulfide bond of EW-H was cleaved, and then nucleophilic addition took place intramolecularly to release the fluorophore (at 580 nm). The response to H2S, EW-H had high sensitivity (86 nM of the detection limit), large Stokes shift (155 nm) and a fast response time. More importantly, the probe was also applied for bioimaging in HepG(2) cells, indicating its potential applications in biological organism. (C) 2020 Elsevier B.V. All rights reserved.
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