Engineering Inorganic Nanoflares with Elaborate Enzymatic Specificity and Efficiency for Versatile Biofilm Eradication

Nanozyme has emerged as a versatile nanocatalyst yet is constrained with limited catalytic efficiency and specificity for various biomedical applications. Herein, by elaborately integrating the recognition/transduction carbon dots (CDs) with platinum nanoparticles (PtNPs), an exquisite CDs@PtNPs (CPP) nanoflare is engineered as an efficient and substrate-specific peroxidase-mimicking nanozyme for high-performance biosensing and antibacterial applications. The intelligent CPP-catalyzed hydrogen peroxide (H2O2)-generated reactive oxygen species realize the sensitive diagnosis-guided enhanced disinfection of pathogens. Significantly, the CPP nanozyme shows the prominent biofilm eradication and wound healing in vivo by virtue of endogenous H(2)O(2)in acidic infection tissues, which can substantially preclude the annoying antibiotics resistance. A fundamental understanding on the present CPP nanoflare would not only facilitate the advancement of various prospective biocatalysts, but also establish a multifunctional means for versatile biosensing and smart diagnostic applications.