期刊
SMALL
卷 16, 期 44, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.202004535
关键词
electrochemiluminescence; high-sensitivity detection; lateral flow immunoassays; mesoporous silica nanoparticles; troponin I
类别
资金
- Mid-Career Researcher Program of a National Research Foundation (NRF) - Ministry of Science, ICT (MSIT) of Korea [NRF-2017R1A2B3010816]
- BioNano HealthGuard Research Center - MSIT of Korea as Global Frontier Project [NRF-2019M3A6B2060097]
The lateral flow immunosensor (LFI) is a widely used diagnostic tool for biomarker detection; however, its sensitivity is often insufficient for analyzing targets at low concentrations. Here, an electrochemiluminescent LFI (ECL-LFI) is developed for highly sensitive detection of troponin I (TnI) using Ru(bpy)(3)(2+)-loaded mesoporous silica nanoparticles (RMSNs). A large amount of Ru(bpy)(3)(2+)is successfully loaded into the mesoporous silica nanoparticles with excellent loading capacity and shows strong ECL signals in reaction to tripropylamine. Antibody-immobilized RMSNs are applied to detect TnI by fluorescence and ECL analysis after a sandwich immunoassay on the ECL-LFI strip. The ECL-LFI enables the highly sensitive detection of TnI-spiked human serum within 20 min at femtomolar levels (approximate to 0.81 pg mL(-1)) and with a wide dynamic range (0.001-100 ng mL(-1)), significantly outperforming conventional fluorescence detection (>3 orders of magnitude). Furthermore, TnI concentrations in 35 clinical serum samples across a low range (0.01-48.31 ng mL(-1)) are successfully quantified with an excellent linear correlation (R-2 = 0.9915) using a clinical immunoassay analyzer. These results demonstrate the efficacy of this system as a high-performance sensing strategy capable of capitalizing on future point-of-care testing markets for biomolecule detection.
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