期刊
SENSORS
卷 20, 期 17, 页码 -出版社
MDPI
DOI: 10.3390/s20174738
关键词
electrochemical biosensor; electrochemical impedance spectroscopy; kanamycin; thiacalix[4]arene; milk contamination
资金
- Russian Science Foundation [16-13-00005]
- European Union [690898]
- Science Grant Agency VEGA [1/0419/20]
- Russian Science Foundation [19-13-11002] Funding Source: Russian Science Foundation
- Marie Curie Actions (MSCA) [690898] Funding Source: Marie Curie Actions (MSCA)
The determination of antibiotics in food is important due to their negative effect on human health related to antimicrobial resistance problem, renal toxicity, and allergic effects. We propose an impedimetric aptasensor for the determination of kanamycin A (KANA), which was assembled on the glassy carbon electrode by the deposition of carbon black in a chitosan matrix followed by carbodiimide binding of aminated aptamer mixed with oligolactide derivative of thiacalix[4]arene in aconeconfiguration. The assembling was monitored by cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy. In the presence of the KANA, the charge transfer resistance of the inner interface surprisingly decreased with the analyte concentration within 0.7 and 50 nM (limit of detection 0.3 nM). This was attributed to the partial shielding of the negative charge of the aptamer and of its support, a highly porous 3D structure of the surface layer caused by a macrocyclic core of the carrier. The use of electrostatic assembling in the presence of cationic polyelectrolyte decreased tenfold the detectable concentration of KANA. The aptasensor was successfully tested in the determination of KANA in spiked milk and yogurt with recoveries within 95% and 115%.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据