期刊
SENSORS
卷 20, 期 20, 页码 -出版社
MDPI
DOI: 10.3390/s20205773
关键词
food safety; electrochemical sensing; foodborne pathogen; fresh produce; interdigitated electrodes; sensor analytic point solution; SNAPS
资金
- National Institute of Food and Agriculture, Agriculture and Food Research Initiative U.S. Department of Agriculture [2019-05855, 2018-672 67016-27578]
- National Science Foundation [CBET-1805512, 1706817, 1706994, 1756999 1511953, 1512659]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1706994, 1706817] Funding Source: National Science Foundation
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1512659, GRANTS:13923135] Funding Source: National Science Foundation
Irrigation water is a primary source of fresh produce contamination by bacteria during the preharvest, particularly in hydroponic systems where the control of pests and pathogens is a major challenge. In this work, we demonstrate the development of a Listeria biosensor using platinum interdigitated microelectrodes (Pt-IME). The sensor is incorporated into a particle/sediment trap for the real-time analysis of irrigation water in a hydroponic lettuce system. We demonstrate the application of this system using a smartphone-based potentiostat for rapid on-site analysis of water quality. A detailed characterization of the electrochemical behavior was conducted in the presence/absence of DNA and Listeria spp., which was followed by calibration in various solutions with and without flow. In flow conditions (100 mL samples), the aptasensor had a sensitivity of 3.37 +/- 0.21 k omega log-CFU-1 mL, and the LOD was 48 +/- 12 CFU mL(-1) with a linear range of 10(2) to 10(4) CFU mL(-1). In stagnant solution with no flow, the aptasensor performance was significantly improved in buffer, vegetable broth, and hydroponic media. Sensor hysteresis ranged from 2 to 16% after rinsing in a strong basic solution (direct reuse) and was insignificant after removing the aptamer via washing in Piranha solution (reuse after adsorption with fresh aptamer). This is the first demonstration of an aptasensor used to monitor microbial water quality for hydroponic lettuce in real time using a smartphone-based acquisition system for volumes that conform with the regulatory standards. The aptasensor demonstrated a recovery of 90% and may be reused a limited number of times with minor washing steps.
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