期刊
SCIENCE
卷 370, 期 6520, 页码 1089-+出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.abe1502
关键词
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资金
- National Institute of Allergy and Infectious Diseases Center for HIV/AIDS Vaccine Development [UM1 AI144462, R01 AI113867, R01 AI132317, P01 AI110657]
- Bill and Melinda Gates Foundation [OPP1170236]
- Novavax, Inc.
- National Institutes of Health [R01-GM129325, P41-GM103311]
- Office of Cyber Infrastructure and Computational Biology, National Institute of Allergy and Infectious Diseases
Vaccine efforts to combat the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is responsible for the current coronavirus disease 2019 (COVID-19) pandemic, are focused on SARS-CoV-2 spike glycoprotein, the primary target for neutralizing antibodies. We performed cryo-election microscopy and site-specific glycan analysis of one of the leading subunit vaccine candidates from Novavax, which is based on a full-length spike protein formulated in polysorbate 80 detergent. Our studies reveal a stable prefusion conformation of the spike immunogen with slight differences in the S1 subunit compared with published spike ectodomain structures. We also observed interactions between the spike trimers, allowing formation of higher-order spike complexes. This study confirms the structural integrity of the full-length spike protein immunogen and provides a basis for interpreting immune responses to this multivalent nanoparticle immunogen.
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