期刊
SCIENCE
卷 369, 期 6510, 页码 1470-+出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.abb4119
关键词
-
资金
- DFG [HU363/12-1, BE 1814/15-1]
- ERC [ADG 741781 GLOWSOME]
Production of small ribosomal subunits initially requires the formation of a 90S precursor followed by an enigmatic process of restructuring into the primordial pre-40S subunit. We elucidate this process by biochemical and cryo-electron microscopy analysis of intermediates along this pathway in yeast. First, the remodeling RNA helicase Dhr1 engages the 90S pre-ribosome, followed by Utp24 endonuclease-driven RNA cleavage at site A(1), thereby separating the 5'-external transcribed spacer (ETS) from 18S ribosomal RNA. Next, the 5 '-ETS and 90S assembly factors become dislodged, but this occurs sequentially, not en bloc. Eventually, the primordial pre-40S emerges, still retaining some 90S factors including Dhr1, now ready to unwind the final small nucleolar U3-18S RNA hybrid. Our data shed light on the elusive 90S to pre-40S transition and clarify the principles of assembly and remodeling of large ribonucleoproteins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据