期刊
SCIENCE
卷 370, 期 6513, 页码 223-+出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.abb7927
关键词
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资金
- ViceChancellor's Award (Cambridge Commonwealth, European and International Trust)
- Bradfield scholarship
- Australian Nanotechnology Network overseas travel fellowship
- Medical Research Council [MC_UP_120117]
- MRC [MC_UP_1201/17] Funding Source: UKRI
Most information loss in cryogenic electron microscopy (cryo-EM) stems from particle movement during imaging, which remains poorly understood. We show that this movement is caused by buckling and subsequent deformation of the suspended ice, with a threshold that depends directly on the shape of the frozen water layer set by the support foil. We describe a specimen support design that eliminates buckling and reduces electron beam-induced particle movement to less than 1 angstrom. The design allows precise foil tracking during imaging with high-speed detectors, thereby lessening demands on cryostage precision and stability. It includes a maximal density of holes, which increases throughput in automated cryo-EM without degrading data quality. Movement-free imaging allows extrapolation to a three-dimensional map of the specimen at zero electron exposure, before the onset of radiation damage.
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