期刊
RNA BIOLOGY
卷 18, 期 9, 页码 1252-1264出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2020.1830553
关键词
SYNCRIP; microRNA; CLIP; neurogenesis; RNA metabolism
资金
- Deutsche Forschungsgemeinschaft [DFG FI 2157/2-1]
SYNCRIP, a member of the hnRNP family, regulates neuronal development and plasticity. Through its interaction with various mRNAs involved in neurogenesis and neurite outgrowth, SYNCRIP has been found to promote early neuronal differentiation. Additionally, SYNCRIP synergizes with pro-neural miRNAs to enhance its effects on neuronal morphogenesis.
SYNCRIP, a member of the cellular heterogeneous nuclear ribonucleoprotein (hnRNP) family of RNA binding proteins, regulates various aspects of neuronal development and plasticity. Although SYNCRIP has been identified as a component of cytoplasmic RNA granules in dendrites of mammalian neurons, only little is known about the specific SYNCRIP target mRNAs that mediate its effect on neuronal morphogenesis and function. Here, we present a comprehensive characterization of the cytoplasmic SYNCRIP mRNA interactome using iCLIP in primary rat cortical neurons. We identify hundreds of bona fide SYNCRIP target mRNAs, many of which encode for proteins involved in neurogenesis, neuronal migration and neurite outgrowth. From our analysis, the stabilization of mRNAs encoding for components of the microtubule network, such as doublecortin (Dcx), emerges as a novel mechanism of SYNCRIP function in addition to the previously reported control of actin dynamics. Furthermore, we found that SYNCRIP synergizes with pro-neural miRNAs, such as miR-9. Thus, SYNCRIP appears to promote early neuronal differentiation by a two-tier mechanism involving the stabilization of pro-neural mRNAs by direct 3MODIFIER LETTER PRIMEUTR interaction and the repression of anti-neural mRNAs in a complex with neuronal miRISC. Together, our findings provide a rationale for future studies investigating the function of SYNCRIP in mammalian brain development and disease.
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