期刊
RNA BIOLOGY
卷 18, 期 8, 页码 1099-1110出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2020.1827785
关键词
Bacteriophage phi KZ; phage-host interaction; Pseudomonas aeruginosa; transcription start site; dRNA-seq; differential expression
资金
- FWO-fundamental research [11D8920N]
- Vogel Stiftung Dr. Eckernkamp
- European Research Council (ERC) under the European Union's ERC [819800]
- European Research Council (ERC) [819800] Funding Source: European Research Council (ERC)
The study used differential RNA-seq to analyze the transcriptome structure of Pseudomonas aeruginosa jumbo phage phi KZ during host infection, revealing new insights into early viral gene expression. The research also observed changes in host gene expression during the critical metabolism conversion, shedding light on the phage-host interactions at a genome-wide level.
As part of the ongoing renaissance of phage biology, more phage genomes are becoming available through DNA sequencing. However, our understanding of the transcriptome architecture that allows these genomes to be expressed during host infection is generally poor. Transcription start sites (TSSs) and operons have been mapped for very few phages, and an annotated global RNA map of a phage - alone or together with its infected host - is not available at all. Here, we applied differential RNA-seq (dRNA-seq) to study the early, host takeover phase of infection by assessing the transcriptome structure of Pseudomonas aeruginosa jumbo phage phi KZ, a model phage for viral genetics and structural research. This map substantially expands the number of early expressed viral genes, defining TSSs that are active ten minutes after phi KZ infection. Simultaneously, we record gene expression changes in the host transcriptome during this critical metabolism conversion. In addition to previously reported upregulation of genes associated with amino acid metabolism, we observe strong activation of genes with functions in biofilm formation (cdrAB) and iron storage (bfrB), as well as an activation of the antitoxin ParD. Conversely, phi KZ infection rapidly down-regulates complexes IV and V of oxidative phosphorylation (atpCDGHF and cyoABCDE). Taken together, our data provide new insights into the transcriptional organization and infection process of the giant bacteriophage phi KZ and adds a framework for the genome-wide transcriptomic analysis of phage-host interactions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据