期刊
RNA BIOLOGY
卷 18, 期 8, 页码 1124-1135出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2020.1829366
关键词
RNA helicase; ribosome; ribonucleoprotein complex (RNP); ATPase; RNA-protein interaction
资金
- Deutsche Forschungsgemeinschaft [SFB1190]
- University Medical Centre Gottingen
RNA helicase DDX55 is predominantly localized in the nucleoplasm in human cells and associates with pre-ribosomal subunits, playing a crucial role in the maturation of large subunit pre-rRNAs. The C-terminal region of DDX55 is essential for its substrate specificity, recruitment to pre-ribosomes, and may target domain IV during LSU biogenesis.
RNA helicases contribute to diverse aspects of RNA metabolism through their functions in re-arranging RNA structures. Identification of the remodelling targets of RNA helicases is a critical step in elucidating their cellular functions. Here, we show that, in contrast to many other ribosome biogenesis factors, the DExD box ATPase DDX55 predominantly localizes to the nucleoplasm and we identify a nuclear localization signal within the C-terminal region of the protein. DDX55 associates with pre-ribosomal subunits in human cells and is required for maturation of large subunit pre-rRNAs. Interestingly,in vitroanalyses show that DDX55 selectively associates with double-stranded RNA substrates, which also stimulate its ATPase activity, and our data suggest that the C-terminal region of DDX55 contributes to this substrate specificity. The C-terminal region of DDX55 is also necessary for recruitment of the helicase to pre-ribosomes and, usingin vivocrosslinking, we reveal a binding site for DDX55 in helix H62 of the 28S ribosomal RNA. Taken together, these data highlight the importance of the C-terminal region of DDX55 in substrate specificity and recruitment, and identify domain IV as a potential remodelling target of DDX55 during LSU biogenesis.
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