期刊
REPRODUCTIVE SCIENCES
卷 28, 期 2, 页码 447-461出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s43032-020-00297-1
关键词
Folate; Inositol; Nutrition; Gut; Placenta; Pregnancy
资金
- Faculty of Science, Carleton University
- department of Obstetrics and Gynaecology at Mount Sinai Hospital
Maternal malnutrition can alter the abundance of gut microbes and folate/inositol transporters, potentially impacting maternal micronutrient status and affecting fetal outcomes.
Maternal malnutrition and micronutrient deficiencies can alter fetal development. However, the mechanisms underlying these relationships are poorly understood. We used a systems physiology approach to investigate diet-induced effects on maternal gut microbes and folate/inositol transport in the maternal/fetal gut and placenta. Female mice were fed a control diet (CON) diet, undernourished (UN, restricted by 30% of CON intake) or a high-fat diet (HF, 60% kcals fat) during pregnancy to model normal pregnancy, fetal growth restriction or maternal metabolic dysfunction, respectively. At gestational day 18.5, we assessed circulating folate levels by microbiological assay, relative abundance of gut lactobacilli by G3PhyloChip (TM), and folate/inositol transporters in placenta and maternal/fetal gut by qPCR/immunohistochemistry. UN and HF-fed mothers had lower plasma folate concentrations vs. CON. Relative abundances of three lactobacilli taxa were higher in HF vs. UN and CON. HF-fed mothers had higher gut proton coupled folate transporter (Pcft) and reduced folate carrier 1 (Rfc1), and lower sodium myo-inositol co-transporter 2 (Smit2), mRNA expression vs. UN and CON. HF placentae had increased folate receptor beta (Fr beta) expression vs. UN. mRNA expression ofPcft, folate receptor alpha (Fr alpha), andSmit2was higher in gut of HF fetuses vs. UN and CON. Transporter protein expression was not different between groups. Maternal malnutrition alters abundance of select gut microbes and folate/inositol transporters, which may influence maternal micronutrient status and delivery to the fetus, impacting pregnancy/fetal outcomes.
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