期刊
PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL
卷 30, 期 5, 页码 686-696出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.pnsc.2020.09.007
关键词
Neurobiology; Micro-patterning; Inkjet printing; Self-assembling peptides; PC12 cells
资金
- EPSRC [EP/N007174/1, EP/N023579/1]
- Royal Society [RG160662, IE150457]
- Jiangsu specially-appointed professor program
- EPSRC [EP/N007174/1, EP/N023579/1] Funding Source: UKRI
The patterning of neuronal cells and guiding neurite growth are important for neuron tissue engineering and cell-based biosensors. In this paper, inkjet printing has been employed to pattern self-assembled I(3)QGK peptide nanofibers on silk substrates for guiding the growth of neuron-like PC12 cells. Atomic force microscopy (AFM) confirmed the dynamic self-assembly of I(3)QGK into nanofiber structures. The printed self-assembled peptide strongly adheres to regenerated silk fibroin (RSF) substrates through charge-charge interactions. It was observed that in the absence of I(3)QGK, PC12 cells exhibited poor attachment to RSF films, while for RSF surfaces coated or printed with peptide nanofibers, cellular attachment was significantly improved in terms of both cell density and morphology. AFM results revealed that peptide nanofibers can promote the generation of axons and terminal buttons of PC12 cells, indicating that I(3)QGK nanofibers not only promote cellular attachment but also facilitate differentiation into neuronal phenotypes. Inkjet printing allows complex patterning of peptide nanofibers onto RSF substrates, which enabled us to engineer cell alignment and provide an opportunity to direct axonal development in vitro. The live/dead assay showed that printed I(3)QGK patterns exhibit no cytotoxicity to PC12 cells demonstrating potential for future nerve tissue engineering applications.
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