期刊
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
卷 163, 期 -, 页码 14-22出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.pbiomolbio.2020.10.007
关键词
Mre11-Rad50-Nbs1; DNA double Strand break repair; DNA repair; Methyl TROSY; NMR spectroscopy; Allostery
资金
- Cancer Prevention and Research Institute of Texas [D-1876]
- National Institute of General Medical Sciences [RP180553]
- [1R35GM128906]
The Mre11-Rad50-Nbs1/Xrs2 protein complex is crucial in detecting and repairing DNA double strand breaks, but little is known about the transitions between different structural states. Solution state NMR spectroscopy and other biophysical techniques have provided important insights into the structures and dynamics associated with Mre11-Rad50 functions.
The Mre11-Rad50-Nbs1/Xrs2 protein complex plays a pivotal role in the detection and repair of DNA double strand breaks. Through traditional and emerging structural biology techniques, various functional structural states of this complex have been visualized; however, relatively little is known about the transitions between these states. Indeed, it is these structural transitions that are important for Mre11Rad50-mediated DNA unwinding at a break and the activation of downstream repair signaling events. Here, we present a brief overview of the current understanding of the structure of the core Mre11-Rad50 complex. We then highlight our recent studies emphasizing the contributions of solution state NMR spectroscopy and other biophysical techniques in providing insight into the structures and dynamics associated with Mre11-Rad50 functions. (c) 2020 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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