期刊
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
卷 160, 期 -, 页码 79-86出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.pbiomolbio.2020.07.011
关键词
ssRNA phage; QBeta; RNA virus; Viral morphogenesis; Virus assembly; Bacteriophage
资金
- Department of Biochemistry and Biophysics at Texas AM University
- Center for Phage Technology - Texas AgriLife
- Center for Phage Technology - Texas AM University
- NSF [1902392]
- NIH [R21AI137696, P01AI095208]
- Welch Foundation [A-1863]
- Texas A&M Triads for Transformation grant
- TAMU X-Grant Program
- TAMU School of Science Strategic Transformative Research Program
- CST*R Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1902392] Funding Source: National Science Foundation
Single-stranded RNA bacteriophages are small spherical RNA viruses that infect bacteria with retractile pili. Recent breakthroughs in high-resolution structural studies of two ssRNA phages, MS2 and Q beta, and their interaction with the host, E. coli, by cryo-electron microscopy provide insights into their infection cycle and potential therapeutic applications.
Single-stranded RNA bacteriophages (ssRNA phages) are small spherical RNA viruses that infect bacteria with retractile pili. The single positive-sense genomic RNA of ssRNA phages, which is protected by a capsid shell, is delivered into the host via the retraction of the host pili. Structures involved in ssRNA phage infection cycle are essential for understanding the underlying mechanisms that can be used to engineer them for therapeutic applications. This review summarizes the recent breakthroughs in high-resolution structural studies of two ssRNA phages, MS2 and Q beta, and their interaction with the host, E. coli, by cryo-electron microscopy (cryo-EM). These studies revealed new cryo-EM structures, which provide insights into how MS2 and Q beta package the RNA, lyse E. coli, and adsorb to the receptor F-pili, responsible for conjugation. Methodologies described here can be expanded to study other ssRNA phages that target pathogenic bacteria. (C) 2020 Elsevier Ltd. All rights reserved.
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