期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 117, 期 38, 页码 23539-23547出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1921408117
关键词
RNA localization; RNA regulation; localized RNA records
资金
- NIH [GM50942, T32 GM008349]
- E.W. Hopkins fellowship
- NIH diversity supplement
- Wharton and Biochemistry Scholar Fellowships
- Wisconsin Alumni Research Foundation (WARF) Scholarship
RNA movements and localization pervade biology, from embryonic development to disease. To identify RNAs at specific locations, we developed a strategy in which a uridine-adding enzyme is anchored to subcellular sites, where it directly marks RNAs with 3' terminal uridines. This localized RNA recording approach yields a record of RNA locations, and is validated through identification of RNAs localized selectively to the endoplasmic reticulum (ER) or mitochondria. We identify a broad dual localization pattern conserved from yeast to human cells, in which the same battery of mRNAs encounter both ER and mitochondria in both species, and include an mRNA encoding a key stress sensor. Subunits of many multiprotein complexes localize to both the ER and mitochondria, suggesting coordinated assembly. Noncoding RNAs in the course of RNA surveillance and processing encounter both organelles. By providing a record of RNA locations over time, the approach complements those that capture snapshots of instantaneous positions.
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