4.7 Article

Overexpression of ovate family protein 22 confers multiple morphological changes and represses gibberellin and brassinosteroid signalings in transgenic rice

期刊

PLANT SCIENCE
卷 304, 期 -, 页码 -

出版社

ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2020.110734

关键词

Oryza sativa; OVATE family protein; Morphology; Gibberellins; SLR1; Brassinosteroid

资金

  1. Jilin Scientific and Technological Development Program (CN), China [20160204007N Y]
  2. National Natural Science Foundation of China [31701369]

向作者/读者索取更多资源

OVATE family proteins (OFPs) are plant-specific transcription factors that regulate plant growth and development by modulating gibberellic acid (GA) biosynthesis and brassinosteroid (BR) responses. Overexpression of OsOFP22 in rice leads to morphological changes, such as reduced plant height and altered leaf shape. OsOFP22 suppresses GA and BR signal transduction by promoting DELLA protein accumulation, integrating GA and BR to regulate plant growth.
OVATE family proteins (OFPs) are plant-specific transcription factors that regulate plant growth and development. OFPs interact with 3-aa loop extension (TALE) homeodomain proteins and brassinosteroid (BR) signaling components to modulate gibberellic acid (GA) biosynthesis and BR responses. Bioactive GAs are essential in regulating plant organogenesis and organ growth by promoting cell differentiation and elongation. DELLA proteins act as the central repressors of GA-regulated processes and are targeted to be degraded by the 26S proteasome in the presence of GA. We discovered that the rice OFP22 negatively regulates GA and BR signal transduction. OsOFP22 expression was rapidly up-regulated by exogenous GA and BR application, detected predominantly in the calli and spikelets. Overexpression of OsOFP22 conferred multiple morphological phenotypes, including reduced plant height, dark green leaves, and shortened and widened leaves, floral organs and grains. The GA-induced elongation of the second leaf sheath in the seedlings, and alpha-amylase activity in the endosperms were attenuated in transgenic lines overexpressing OsOFP22, while GA-biosynthesis gene transcripts and bioactive GA3 and GA4 contents were increased in the transgenic plants. OsOFP22 promotes the protein accumulation of SLR1, the single DELLA in rice protein. Furthermore, Overexpression of OsOFP22 suppresses BR response and the expression of BR-related genes. OsOFP22 is thus involved in the repression of GA and BR signal transduction and integrates GA with BR to regulate plant growth and development.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据