期刊
PLANT PHYSIOLOGY
卷 184, 期 4, 页码 1717-1730出版社
AMER SOC PLANT BIOLOGISTS
DOI: 10.1104/pp.20.01219
关键词
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资金
- National Science Foundation [IOS-1555581, EPS 0236913, MCB 1413036, MCB 0920663, DBI 0521587, DBI 1228622]
- U.S. Department of Agriculture, Prosser Irrigated Agricultural Research Center at Washington State University (Hatch umbrella project) [1015621]
- Kansas Technology Enterprise Corporation
- K-IDeA Networks of Biomedical Research Excellence of the National Institutes of Health [P20GM103418]
- Kansas State University
Arabidopsis fab1 mutants die at 2 degrees C; however, reducing levels of high-melting-point phosphatidylglycerol species through expression of a chloroplast-targeted 16:0 desaturase rescues fab1 plants. The Arabidopsis (Arabidopsis thaliana) fatty acid biosynthesis1 (fab1) mutant has increased levels of the saturated fatty acid 16:0, resulting from decreased activity of 3-ketoacyl-ACP synthase II. In fab1 leaves, phosphatidylglycerol, the major chloroplast phospholipid, contains >40% high-melting-point molecular species (HMP-PG; molecules that contain only 16:0, 16:1-trans, and 18:0 fatty acids)-a trait associated with chilling-sensitive plants-compared with <10% in wild-type Arabidopsis. Although they do not exhibit short-term chilling sensitivity when exposed to low temperatures (2C to 6 degrees C) for long periods, fab1 plants do suffer collapse of photosynthesis, degradation of chloroplasts, and eventually death. To test the relevance of HMP-PG to the fab1 phenotype, we used transgenic 16:0 desaturases targeted to the endoplasmic reticulum and the chloroplast to lower 16:0 in leaf lipids of fab1 plants. We produced two lines that had very similar lipid compositions except that one, ER-FAT5, contained high HMP-PG, similar to the fab1 parent, while the second, TP-DES9*, contained <10% HMP-PG, similar to the wild type. TP-DES9* plants, but not ER-FAT5 plants, showed strong recovery and growth following 75 d at 2C, demonstrating the role of HMP-PG in low-temperature damage and death in fab1, and in chilling-sensitive plants more broadly.
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