Ecology-based analysis of a recent association between Spartium junceum and 16SrV phytoplasma

An outbreak of Spartium witches' broom (SpaWB) in Sicily prompted us to identify and characterize associated phytoplasmas. Over 80 samples of Spanish broom (Spartium junceum) and around 270 individuals of the potential vector Livilla spectabilis were collected and analysed. Single and mixed infections of 16SrV and 'Candidatus Phytoplasma spartii' were detected in Spanish broom samples and for the first time in L. spectabilis. The 16SrV isolates were further characterized by multilocus sequence typing (MLST) to determine their phylogenetic relationship with flavescence doree phytoplasma (FDp) and to evaluate the risk of host-jumping to grapevine. Phylogenetic analysis of most of the analysed genes using the MLST approach grouped S. junceum 16SrV-C isolates with FDp isolates infecting grapevine and Scaphoideus titanus. Notably, phylogenetic analysis of the vmpA gene clustered the S. junceum isolates with FDp genotypes transmitted by S. titanus. This study extends the knowledge of SpaWB epidemiology, focusing on the possible risk of a 16SrV host jump from Spanish broom to grapevine. Spanish broom was identified as a reservoir and potential inoculum source of phytoplasmas that cause severe disease in cultivated crops. Furthermore, the L. spectabilis psyllid may be involved in the epidemiology of this 16SrV-C phytoplasma, although in the absence of in vivo transmission trials. The study further confirms the strong ability of phytoplasmas to adapt to new hosts and vectors, thus leading to potential phytosanitary emergencies.

Automated summary

The outbreak of Spartium witches' broom (SpaWB) in Sicily led to the identification and characterization of associated phytoplasmas. Analysis of Spanish broom samples and Livilla spectabilis individuals revealed single and mixed infections of 16SrV and 'Candidatus Phytoplasma spartii', with potential host-jumping risk to grapevine. Phylogenetic analysis indicated a strong ability of phytoplasmas to adapt to new hosts and vectors, posing potential phytosanitary emergencies.