期刊
PLANT DISEASE
卷 105, 期 4, 页码 1006-1012出版社
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/PDIS-03-20-0572-RE
关键词
Geminivirus; high-throughput sequencing; nightshade
资金
- National Key Research and Development Programs of China [2018YFC0809200]
- Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University [HUZUL201906]
- Science and Technology Program of Hangzhou [20190101A01]
A novel geminivirus, provisionally designated nightshade curly top virus (NCTV), was identified in Zhejiang Province, China, causing virus-like symptoms on nightshade plants. The virus is widely distributed and highly infectious to various plant species, suggesting it belongs to the genus Topocuvirus.
Virus-like symptoms, including leaf deformation and curling, were observed on nightshade (Solanum nigrum) in Zhejiang Province, China. To identify possible pathogenic viruses or viroids, a symptomatic sample was subjected to deep sequencing of small interfering RNAs. Assembly of the resulting sequences led to identification of a novel geminivirus, provisionally designated nightshade curly top virus (NCTV). The complete genomic DNA sequence is 2,867 nucleotides and encodes seven open reading frames. NCTV shares 77.1% overall nucleotide sequence identity, 86.3% coat protein amino acid identity, and 78.9% replication-associated protein amino acid sequence identity with Tomato pseudo-curly top virus, a member of the genus Topocuvirus. PCR screening of nightshade field isolates indicated that NCTV is widely distributed in Zhejiang. Agrobacte-rium-mediated inoculation revealed that NCTV is highly infectious to Nicotiana benthamiana, S. nigrum, S. lycopersicum, and S. tuberosum. Based on pairwise comparisons and phylogenetic analyses, NCTV is proposed as a provisional member of the genus Topocuvirus.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据