期刊
PLANT CELL TISSUE AND ORGAN CULTURE
卷 143, 期 3, 页码 487-497出版社
SPRINGER
DOI: 10.1007/s11240-020-01934-y
关键词
Pisum; Anther culture; Wild relatives; Androgenic response; Plant regeneration
资金
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET, Argentina)
- Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR-CONICET)
- Fondo para la Investigacion Cientifica y Tecnologica (FONCyT, Argentina)
In pea breeding is important to shorten the generation cycles to obtain homozygosity quickly. Doubled haploid technology is important to attain this purpose and androgenesis is the most promising tool for induction of haploids in legumes. Commercial pea varieties have been described as recalcitrant to this approach but very little is known regarding the androgenic competence of pea relatives. In this work, a comparative study of the androgenic response among different taxa of the genusPisumwas undertaken.We cultured anthers of 11 pea materials from the primary and secondary genepools under the same experimental conditions, and studied their competence to produce calli and plants in vitro. Significant differences were found in the percentage of callus and plant production between the different species and subspecies. The two wild formsPisum fulvumSibth. & Sm. andPisum sativumsubsp.elatius(Bieb.) Aschers. & Graebn. regenerated shoots from anther culture with the highest efficiency (67% and 38%, respectively), becoming potential sources of androgenic competence. Among the cultivated genotypes ofP. sativum, the botanical varietyarvenseregenerated shoots with the highest percentage (40%) also being a good candidate to study androgenesis. The commercial varieties tested showed significant differences in the callus and plant production, with Primogenita (FCA-INTA) and B101 giving the best results although with low plant regeneration percentages (17% and 11%, respectively).P. fulvum,P. sativumsubsp. elatiusandP. sativumsubspsativumvararvensewere identified as highly responsive to anther culture, useful to transfer androgenesis competence to recalcitrant commercial varieties. Key message Within genusPisum, the wild formsP.fulvum,P. sativumsubspecieselatiusand the cultivated varietyarvensewere identified as potential sources to introduce androgenesis competence into recalcitrant commercial varieties.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据