期刊
PLANT CELL TISSUE AND ORGAN CULTURE
卷 144, 期 1, 页码 35-48出版社
SPRINGER
DOI: 10.1007/s11240-020-01884-5
关键词
Dormant budwood; Clonal genebanking; Applied cryopreservation; Tree fruit germplasm; Liquid nitrogen storage
资金
- United States Department of Agriculture-Agricultural Research Service (USDA-ARS) [58-3012-5-010]
- Colorado State University Department of Horticulture and Landscape Architecture (CSU HLA) [58-3012-5-010]
- National Academy of Sciences (NAS)
- USAID
Dormant bud cryopreservation is an efficient method for preserving important agronomic traits in deciduous trees and shrubs, utilizing the natural dormancy of cold hardy crops. It reduces labor requirements, saves resources, and presents challenges in seasonal work.
Key message This review aims to provide information about the DB cryopreservation method with a focus on its many variables and permutations reported in literature to allow genebanks and germplasm managers to make informed decisions regarding the designation of critical resources. Cryopreservation of clonal plant germplasm is a reliable way to preserve important agronomic traits and protect against loss of crop genetic diversity of many horticultural species. Dormant bud cryopreservation techniques present an efficient alternative to the labor-intensive shoot tip cryopreservation process and may allow a single technician to preserve large quantities of germplasm in a season. This method of cryopreservation takes advantage of the natural dormancy in cold hardy crops, making it a viable technique for only deciduous trees and shrubs. Many factors must be considered when attempting to perform dormant bud research methods to applied-level germplasm preservation efforts. This process is necessarily a seasonal endeavor, which puts strain on labor and facilities particularly in winter. Integration of methods and procedures using different crop species or new equipment provides additional challenges that must be tested in advance. By identifying variables of dormant bud processing in cryopreservation literature, options emerge that allow for the modification of reported methods to work within the confines of institutional resources. Infrastructure, pre-processing, and recovery stages are discussed in terms of necessity and available alternatives to allow informed decision making in establishing an applied dormant budwood genebank.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据