Phytochrome A and its Functional Manifestations in Etiolated and Far-red Light-grown Seedlings of the Wild-type Rice and its Hebiba and Cpm2 Mutants Deficient in the Defense-related Phytohormone Jasmonic Acid

Interaction between phytochromes and hormones is becoming one of the major issues in plant photophysiology. In this work, effects of defense-related jasmonic acid (JA) on phytochrome A (phyA) were investigated by fluorescence spectroscopy making use of two JA biosynthesis mutants of rice: cpm2 with the inactivated gene allene oxide cyclase and hebiba with additional genes deleted. Constant far-red light (FRc) mediated by phyA reduced its content in the wild type (WT) and mutants, and brought about domination of its light-stable pool (phyA '') in WT and light-labile pool (phyA ') in the mutants. Pulsed FRp was much less effective. This FR effect classifies as primarily HIR with a low fluence threshold; it comprises inhibition of phyA biosynthesis, stimulation of phyA ''-> phyA ' transformation and phyA ' destruction. In the mutants, phyA suppresses [Pchlide] under FRp (VLFR) and stimulates it under FRc (HIR); these effects are lacking in WT. Similarly, phyA suppresses roots'growth under FRp in the mutants but not in WT. These JA mutant features suggest that JA reduces the phyA functional activity primarily in its phyA '' form mediating HIR. This modulating JA action on phyA functions under FR limiting their extreme manifestations may have contributed to the evolutionary advances of the land plants.

Automated summary

The study investigated the effects of defense-related jasmonic acid (JA) on phytochrome A (phyA) in rice, showing that JA primarily reduces the functional activity of phyA mainly in its phyA '' form mediating high irradiance response. Far-red light results in a decrease in phyA content and different effects in WT and mutants.