期刊
PARASITOLOGY RESEARCH
卷 119, 期 11, 页码 3909-3913出版社
SPRINGER
DOI: 10.1007/s00436-020-06887-x
关键词
Trichomonas gallinae; Real-time PCR; SYBR green; Trichomoniasis; Flagellates
类别
资金
- Projekt DEAL
- Sudanese Ministry of Higher Education and Scientific Research
- Vietnamese Government Scholarship
Trichomonas gallinaeare parasitic flagellates of importance in wild and domestic birds. The parasite is worldwide distributed, and Columbine birds are its main host. Current research focuses mostly on epidemiological and phylogenetic studies. However, there is still a lack of knowledge regarding parasite-host interaction or therapy development. Real-time PCR is a useful tool for diagnostic and quantification of gene copies in a determined sample. By amplification of a 113-bp region of the 18S small subunit ribosomal RNA gene, a SYBR green-based real-time PCR assay was developed. A standard curve was prepared for quantification analysis. Assay efficiency, linearity, and dissociation analysis were successfully performed. Specificity, sensibility, and reproducibility analysis were tested. This assay could be a useful tool not only for diagnostic purposes but also for future in vivo and in vitroT. gallinaestudies.
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