期刊
NUCLEIC ACIDS RESEARCH
卷 48, 期 19, 页码 11113-11129出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkaa860
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资金
- Ministry of Science and Technology of Zhejiang Province [2018C03026]
- National Key Research and Devlopment Program of China [2018YFC1004802]
- National Basic Research Priorities Program of China [2014CB541704]
- National Natural Science Foundation of China [82030028, 81670932]
- TheKey Projects of Shangdong Provincial Programs for Research and Development [2017CXGC1213]
In this report, we investigated the molecular mechanism underlying a deafness-associated m.7516delA mutation affecting the 5' end processing sites of mitochondrial tRNA(AsP) and tRNA(Ser(UCN)). An in vitro processing experiment demonstrated that m.7516delA mutation caused the aberrant 5' end processing of tRNA(Ser(UCN)) and tRNA(AsP) precursors, catalyzed by RNase P. Using cytoplasmic hybrids (cybrids) derived from one hearing-impaired Chinese family bearing the m.7516delA mutation and control, we demonstrated the asymmetrical effects of m.7516delA mutation on the processing of tRNAs in the heavy (H)-strand and light (L)-strand polycistronic transcripts. Specially, the m.7516delA mutation caused the decreased levels of tRNA(Ser(UCN)) and downstream five tRNAs, including tRNA(Tyr) from the L-strand transcripts and tRNA(AsP) from the H-strand transcripts. Strikingly, mutant cybrids exhibited the lower level of COX2 mRNA and accumulation of longer and uncleaved precursors of COX2 from the H-strand transcripts. Aberrant RNA metabolisms yielded variable reductions in the mitochondrial proteins, especially marked reductions in the levels of ND4, ND5, CO1, CO2 and CO3. The impairment of mitochondria, translation caused the proteostasis stress and respiratory deficiency, diminished ATP production and membrane potential, increased production of reactive oxygen species and promoted apoptosis. Our findings provide new insights into the pathophysiology of deafness arising from mitochondrial tRNA processing defects.
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