期刊
NEW PHYTOLOGIST
卷 229, 期 3, 页码 1298-1311出版社
WILEY
DOI: 10.1111/nph.16937
关键词
carbon assimilation; crop improvement; food security; heat stress; photosynthesis; Rubisco activase; Rubisco regulation; Triticum aestivum(wheat)
资金
- Lancaster Environment Centre
- Biotechnology and Biological Sciences Research Council through the International Wheat Yield Partnership project 'Speeding the adjustment of photosynthesis to shade-sun transitions to increase yield potential in the field' [IWYP163, BB/S005072/1]
- Biotechnology and Biological Sciences Research Council through the International Wheat Yield Partnership project 'Manipulating stomatal blue light response in wheat to improve productivity' [IWYP123, BB/S005080/1]
- BBSRC [BB/S005072/1, BB/S005080/1] Funding Source: UKRI
The wheat genome encodes three isoforms of Rubisco activase with different thermostability, which can be utilized to enhance the crop's resilience to global warming. High temperatures lead to rapid changes in Rca gene expression and adaptive adjustments in the abundance of Rca isoforms in plants.
TheTriticum aestivum(wheat) genome encodes three isoforms of Rubisco activase (Rca) differing in thermostability, which could be exploited to improve the resilience of this crop to global warming. We hypothesized that elevated temperatures would cause an increase in the relative abundance of heat-stable Rca1 beta. Wheat plants were grown at 25 degrees C : 18 degrees C (day : night) and exposed to heat stress (38 degrees C : 22 degrees C) for up to 5 d at pre-anthesis. Carbon (C) assimilation, Rubisco activity, CA1Pase activity, transcripts of Rca1 beta, Rca2 beta, and Rca2 alpha, and the quantities of the corresponding protein products were measured during and after heat stress. The transcript ofRca1 beta increased 40-fold in 4 h at elevated temperatures and returned to the original level after 4 h upon return of plants to control temperatures. Rca1 beta comprised up to 2% of the total Rca protein in unstressed leaves but increased three-fold in leaves exposed to elevated temperatures for 5 d and remained high at 4 h after heat stress. These results show that elevated temperatures cause rapid changes inRcagene expression and adaptive changes in Rca isoform abundance. The improved understanding of the regulation of C assimilation under heat stress will inform efforts to improve wheat productivity and climate resilience.
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