期刊
NATURE METHODS
卷 17, 期 9, 页码 923-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41592-020-0914-9
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资金
- National Institutes of Health [GM063210, R01-GM080139]
- Wellcome Trust [20947/Z/17/Z]
- Phenix Industrial Consortium
- US Department of Energy [DE-AC02-05CH11231]
A density-modification procedure for improving maps from single-particle electron cryogenic microscopy (cryo-EM) is presented. The theoretical basis of the method is identical to that of maximum-likelihood density modification, previously used to improve maps from macromolecular X-ray crystallography. Key differences from applications in crystallography are that the errors in Fourier coefficients are largely in the phases in crystallography but in both phases and amplitudes in cryo-EM, and that half-maps with independent errors are available in cryo-EM. These differences lead to a distinct approach for combination of information from starting maps with information obtained in the density-modification process. The density-modification procedure was applied to a set of 104 datasets and improved map-model correlation and increased the visibility of details in many of the maps. The procedure requires two unmasked half-maps and a sequence file or other source of information on the volume of the macromolecule that has been imaged. A method based on maximum likelihood density modification, adapted from X-ray crystallography, improves cryo-EM maps.
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