期刊
NATURAL PRODUCT RESEARCH
卷 36, 期 9, 页码 2215-2222出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786419.2020.1826477
关键词
Biosynthesis; heliolactone; methyl carlactonoate; strigolactone; sunflower
资金
- JST/JICA SATREPS [JPMJSA1607]
- JSPS KAKENHI [19K05840 and19H02897]
Heliolactone is a non-canonical strigolactone isolated from sunflower root exudates. This study provides evidence that (11R)-MeCLA is the biosynthetic precursor of heliolactone and suggests the involvement of cytochrome P450-dependent monooxygenation in the conversion of heliolactone to an unidentified metabolite.
Heliolactone is a non-canonical strigolactone isolated from sunflower root exudates. We have previously demonstrated that exogenously administered carlactonoic acid (CLA) was converted to heliolactone in sunflower. The conversion of CLA to heliolactone requires the methyl esterification of the carboxylic acid at C-19. Also, the CLA conversion to its methyl ester, methyl carlactonoate (MeCLA), was demonstrated by feeding experiment in sunflower. However, the involvement of MeCLA in heliolactone biosynthesis remains unclear. We synthesised MeCLA in its racemic form and resolved it into its enantiomers. Feeding experiments revealed that (11R)-MeCLA was exclusively converted to heliolactone in sunflower. This result is an evidence that (11R)-MeCLA is the biosynthetic precursor of heliolactone. Further conversion of heliolactone to an unidentified metabolite with a molecular mass larger than heliolactone by 16 Da was confirmed. The conversion was inhibited by a cytochrome P450 inhibitor, suggesting the involvement of cytochrome P450-dependent monooxygenation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据