期刊
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
卷 29, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.nano.2020.102268
关键词
Nano-bio interface; Biofouling; Chimera protein; Electrostatic interaction; Biotechnology
资金
- Spanish MINECO
- Instituto de Salud Carlos III
- European Union FEDER funds [PI16/00496, PI19/00349, DTS19/00033, CTM2014-58481-R, CTM201784050-R, PGC2018-101464-B-I00, FPU15/06881]
- NanoBioApp Network [MINECO-17-MAT2016-81955-REDT]
- Xunta de Galicia (Centro Singular de Investigacion de Galicia, Accreditation 2016-2019)
- Xunta de Galicia [EM2014/035]
- European Union (European Regional Development Fund-ERDF)
- IDIVAL [PREVAL16/02, PREVAL16/03, INNVAL 17/11, INNVAL 18/28]
Here we propose a one-step strategy to endow nanomaterials with a custom-designed bio-identity. This study designs a universal 'nanomaterial binding domain' that can be genetically attached to any protein ensuring precise and spontaneous protein orientation. We demonstrate how, despite the simplicity of the method, the bioconjugation achieved: (i) is highly efficient, even in the presence of competing proteins, (ii) is stable at extreme physiological conditions (pH ranges 5.2-9.0; NaCl concentrations 0-1 M); (iii) prevents unwanted protein biofouling days after incubation in biologically-relevant conditions; and finally, (iv) avoids nanoparticle interaction with promiscuous unspecific receptors. In summary, this protein biocoating technique, applicable to a wide array of nano-designs, integrates material science and molecular biology procedures to create hybrid nanodevices with well-defined surfaces and predictable biological behaviors, opening a chapter in precision nanodiagnostics, nanosensing or nanotherapeutic applications. (C) 2020 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据