期刊
NANOMEDICINE
卷 15, 期 20, 页码 1965-1980出版社
FUTURE MEDICINE LTD
DOI: 10.2217/nnm-2020-0056
关键词
delivery tool; exosome; infertility; reproductive medicine; sperm
资金
- Ministry of Science, Innovation and Universities, Spain [RYC-2014-15581, AGL2016-81890-REDT]
- Regional Government of Catalonia, Spain [2017-SGR-1229]
- Nuffield Department of Women's and Reproductive Health, University of Oxford, UK
- Wellcome Trust [100262Z/12/Z]
- Kennedy Trust for Rheumatology Research for Imaging facilities
- European Commission [ERC-2014-AdG 670930]
Aim:To investigate exosomes as a noninvasive delivery tool for mammalian sperm.Materials & Methods:Exosomes were isolated from HEK293T cells and co-incubated with boar spermin vitro.Results: Internalised exosomes were detected within 10 min of co-incubation. Computer-assisted sperm analysis and flow cytometry demonstrated that even after 5-h of exposure to exosomes, there were no significant deleterious effects with regard to sperm motility, viability, membrane integrity and mitochondrial membrane potential (p > 0.05), thus indicating that exosomes did not interfere with basic sperm function.Conclusion:HEK293T-derived exosomes interacted with boar sperm without affecting sperm function. Exosomes represent a versatile and promising research tool for studying sperm biology and provide new options for the diagnosis and treatment of male infertility.
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