期刊
NANO LETTERS
卷 20, 期 10, 页码 7655-7661出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.0c03189
关键词
surface-enhanced Raman spectroscopy (SERS); infectious disease; gold nanorods; bacteria; cryo-electron microscopy
类别
资金
- Stanford Catalyst for Collaborative Solutions [132114]
- Gates Foundation [OPP 1113682]
- National Science Foundation [ECCS-1542152, 1905209]
- Center for Cancer Nanotechnology Excellence and Translation grant from the NCI-NIH [1U54CA199075]
- NIH [NCI 1R01CA199656-01A1, 1R01 CA222836-01A1, T32 CA009695]
- National Institutes of Health (NIH) K99/R00 Pathway to Independence award [1K99CA234208-01A1]
- Marie Sklodowska-Curie MINDED Project [754490]
- NIH Biotechnology Training Program [T32GM008412]
- Agilent
- Stanford EDGE
- Stanford DARE graduate fellowships
- Marie Curie Actions (MSCA) [754490] Funding Source: Marie Curie Actions (MSCA)
- Division Of Materials Research
- Direct For Mathematical & Physical Scien [1905209] Funding Source: National Science Foundation
Surface-enhanced Raman spectroscopy (SERS) is a promising cellular identification and drug susceptibility testing platform, provided it can be performed in a controlled liquid environment that maintains cell viability. We investigate bacterial liquid-SERS, studying plasmonic and electrostatic interactions between gold nanorods and bacteria that enable uniformly enhanced SERS. We synthesize five nanorod sizes with longitudinal plasmon resonances ranging from 670 to 860 nm and characterize SERS signatures of Gram-negative Escherichia coli and Serratia marcescens and Gram-positive Staphylococcus aureus and Staphylococcus epidermidis bacteria in water. Varying the concentration of bacteria and nanorods, we achieve large-area SERS enhancement that is independent of nanorod resonance and bacteria type; however, bacteria with higher surface charge density exhibit significantly higher SERS signal. Using cryo-electron microscopy and zeta potential measurements, we show that the higher signal results from attraction between positively charged nanorods and negatively charged bacteria. Our robust liquid-SERS measurements provide a foundation for bacterial identification and drug testing in biological fluids.
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