期刊
MOLECULES
卷 25, 期 20, 页码 -出版社
MDPI
DOI: 10.3390/molecules25204675
关键词
NMR-compatible bioreactor; automated NMR; bioprocess monitoring; microbial bioprocess; waste degradation
资金
- project BioPEC - Federal Ministry of Education and Research (BMBF), Germany [01DQ17014]
- project BioPEC - Department of Biotechnology (DBT), India [BT/IN/BMBF-Germany/29/SKM/2016-17]
- KIT-Publication Fund of the Karlsruhe Institute of Technology
We present a Nuclear Magnetic Resonance (NMR) compatible platform for the automated real-time monitoring of biochemical reactions using a flow shuttling configuration. This platform requires a working sample volume of similar to 11 mL and it can circulate samples with a flow rate of 28 mL/min, which makes it suitable to be used for real-time monitoring of biochemical reactions. Another advantage of the proposed low-cost platform is the high spectral resolution. As a proof of concept, we acquire 1H NMR spectra of waste orange peel, bioprocessed using Trichoderma reesei fungus, and demonstrate the real-time measurement capability of the platform. The measurement is performed over more than 60 h, with a spectrum acquired every 7 min, such that over 510 data points are collected without user intervention. The designed system offers high resolution, automation, low user intervention, and, therefore, time-efficient measurement per sample.
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