期刊
MOLECULAR THERAPY
卷 28, 期 12, 页码 2691-2702出版社
CELL PRESS
DOI: 10.1016/j.ymthe.2020.10.001
关键词
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资金
- Mercator Foundation, Germany [St-2018-0014]
- BMBF e:KID [01ZX1612A]
- BMBF NoChro [FKZ 13GW0338B]
- SepsisDataNet [EFRE-0800984]
Preventing the progression to acute respiratory distress syndrome (ARDS) in COVID-19 is an unsolved challenge. The involvement of T cell immunity in this exacerbation remains unclear. To identify predictive markers of COVID-19 progress and outcome, we analyzed peripheral blood of 10 COVID-19-associated ARDS patients and 35 mild/moderate COVID-19 patients, not requiring intensive care. Using multi-parametric flow cytometry, we compared quantitative, phenotypic, and functional characteristics of circulating bulk immune cells, as well as SARS-CoV-2 S-protein-reactive T cells between the two groups. ARDS patients demonstrated significantly higher S-protein-reactive CD4(+) and CD8(+) T cells compared to non-ARDS patients. Of interest, comparison of circulating bulk T cells in ARDS patients to non-ARDS patients demonstrated decreased frequencies of CD4(+) and CD8(+) T cell subsets, with activated memory/effector T cells expressing tissue migration molecule CD11a(++). Importantly, survival from ARDS (4/10) was accompanied by a recovery of the CD11a(++) T cell subsets in peripheral blood. Conclusively, data on S-protein-reactive polyfunctional T cells indicate the ability of ARDS patients to generate antiviral protection. Furthermore, decreased frequencies of activated memory/effector T cells expressing tissue migratory molecule CD11a(++) observed in circulation of ARDS patients might suggest their involvement in ARDS development and propose the CD11a-based immune signature as a possible prognostic marker.
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