Ribosomal protein QM/RPL10 positively regulates defence and protein translation mechanisms during nonhost disease resistance

Ribosomes play an integral part in plant growth, development, and defence responses. We report here the role of ribosomal protein large (RPL) subunit QM/RPL10 in nonhost disease resistance. TheRPL10-silencedNicotiana benthamianaplants showed compromised disease resistance against nonhost pathogenPseudomonas syringaepv.tomatoT1. The RNA-sequencing analysis revealed that many genes involved in defence and protein translation mechanisms were differentially affected due to silencing ofNbRPL10.Arabidopsis AtRPL10RNAi andrpl10mutant lines showed compromised nonhost disease resistance toP. syringaepv.tomatoT1 andP. syringaepv.tabaci. Overexpression ofAtRPL10AinArabidopsisresulted in reduced susceptibility against host pathogenP. syringaepv.tomatoDC3000. RPL10 interacts with the RNA recognition motif protein and ribosomal proteins RPL30, RPL23, and RPS30 in the yeast two-hybrid assay. Silencing or mutants of genes encoding these RPL10-interacting proteins inN. benthamianaorArabidopsis, respectively, also showed compromised disease resistance to nonhost pathogens. These results suggest that QM/RPL10 positively regulates the defence and translation-associated genes during nonhost pathogen infection.