4.6 Article

Leptin Contributes to Neuropathic Pain via Extrasynaptic NMDAR-nNOS Activation

期刊

MOLECULAR NEUROBIOLOGY
卷 58, 期 3, 页码 1185-1195

出版社

SPRINGER
DOI: 10.1007/s12035-020-02180-1

关键词

Neuropathic pain; Leptin; NMDAR; Synaptic; Extrasynaptic

资金

  1. National Natural Science Foundation of China [31100806, 81771484, 82073340]
  2. Natural Science Foundation of Guangdong Province, China [2018A030313835]
  3. Program for Changjiang Scholars and Innovative Research Team in University [IRT_16R37]

向作者/读者索取更多资源

This study investigated the role of leptin in neuropathic pain and found that leptin promotes neuropathic pain by enhancing NR2B-mediated currents and nNOS expression. The data suggest an important cellular link between the spinal effects of leptin and the extrasynaptic NMDAR-nNOS-mediated cellular mechanism of neuropathic pain.
Leptin is an adipocytokine that is primarily secreted by white adipose tissue, and it contributes to the pathogenesis of neuropathic pain in collaboration with N-methyl-D-aspartate receptors (NMDARs). Functional NMDARs are a heteromeric complex that primarily comprise two NR1 subunits and two NR2 subunits. NR2A is preferentially located at synaptic sites, and NR2B is enriched at extrasynaptic sites. The roles of synaptic and extrasynaptic NMDARs in the contribution of leptin to neuropathic pain are not clear. The present study examined whether the important role of leptin in neuropathic pain was related to synaptic or extrasynaptic NMDARs. We used a rat model of spared nerve injury (SNI) and demonstrated that the intrathecal administration of the NR2A-selective antagonist NVP-AAM077 and the NR2B-selective antagonist Ro25-6981 prevented and reversed mechanical allodynia following SNI. Administration of exogenous leptin mimicked SNI-induced behavioral allodynia, which was also prevented by NVP-AAM077 and Ro25-6981. Mechanistic studies showed that leptin enhanced NR2B- but not NR2A-mediated currents in spinal lamina II neurons of naive rats. Leptin also upregulated the expression of NR2B, which was blocked by the NR2B-selective antagonist Ro25-6981, in cultured dorsal root ganglion (DRG) neurons. Leptin enhanced neuronal nitric oxide synthase (nNOS) expression, which was also blocked by Ro25-6981, in cultured DRG cells. However, leptin did not change NR2A expression, and the NR2A-selective antagonist NVP-AAM077 had no effect on leptin-enhanced nNOS expression. Our data suggest an important cellular link between the spinal effects of leptin and the extrasynaptic NMDAR-nNOS-mediated cellular mechanism of neuropathic pain.

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