期刊
MOLECULAR MICROBIOLOGY
卷 114, 期 6, 页码 979-990出版社
WILEY
DOI: 10.1111/mmi.14588
关键词
biofilms; extracellular matrix; marine bacteria; Pseudoalteromonas tunicata; S-layer
资金
- Natural Sciences and Engineering Research Council of Canada [RGPAS-2019-00004, RGPIN-2019-04266]
S-layers are paracrystalline proteinaceous lattices that surround prokaryotic cells, forming a critical interface between the cells and their extracellular environment. Here, we report the discovery of a novel S-layer protein present in the Gram-negative marine organism,Pseudoalteromonas tunicataD2. An uncharacterized protein (EAR28894) was identified as the most abundant protein in planktonic cultures and biofilms. Bioinformatic methods predicted a beta-helical structure for EAR28894 similar to theCaulobacterS-layer protein, RsaA, despite sharing less than 20% sequence identity. Transmission electron microscopy revealed that purified EAR28894 protein assembled into paracrystalline sheets with a unique square lattice symmetry and a unit cell spacing of similar to 9.1 nm. An S-layer was found surrounding the outer membrane in wild-type cells and completely removed from cells in an EAR28894 deletion mutant. S-layer material also appeared to be shed from wild-type cells and was highly abundant in the extracellular matrix where it is associated with outer membrane vesicles and other matrix components. EAR28894 and its homologs form a new family of S-layer proteins that are widely distributed in Gammaproteobacteria including species ofPseudoalteromonasandVibrio, and found exclusively in marine metagenomes. We propose the name Slr4 for this novel protein family.
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