期刊
MOLECULAR BIOLOGY
卷 54, 期 4, 页码 520-534出版社
PLEIADES PUBLISHING INC
DOI: 10.1134/S0026893320040093
关键词
cancer; testis antigens; gene expression; copy number; DNA methyltransferases; methylation; retrotransposons; colorectal cancer; microRNA
资金
- Ministry of Health of the Russian Federation [AAAA-A18-118072790013-1]
The transcriptional activity of genes encoding cancer/testis antigens (CTA) and its regulation in colorectal cancer (CRC) is not well understood. The expression of CTA coding genes (CT genes) and possible mechanisms for its regulation, including expression and copy number of DNA methyltransferase genes, copy number of CT genes, microRNA expression, and LINE-1 methylation in CRC were analyzed in this study. The relative expression levels and copy number variation of 19 genes,MAGE-A1,-A2,-A3,-A4,-B1,-B2,GAGE-1,-3,-4,MAGEC1,BAGE,XAGE3,NY-ESO1,SSX2,SCP1,PRAME1,DNMT1,DNMT3A, andDNMT3B, were determined using real-time quantitative PCR. Quantitative methylation of LINE-1 CpG sites was evaluated by pyrosequencing, and multiple parallel sequencing was used to determine the level of microRNA expression. It was found that in colon tumor tissue a multidirectional destabilization of the transcriptional activity ofDNMT3AandDNMT3B, associated with copy number variation and a change in expression of the CT genesBAGE,SSX2andPRAME1, is observed. A strong positive correlation was found between copy number and expression of theBAGE,SSX2, andPRAME1genes. As a result of multiple parallel sequencing, 6 differentially expressed microRNAs (hsa-miR-143-3p, hsa-miR-26a-5p, hsa-miR-25-3p, hsa-miR-92a-3p, hsa-miR-21-5p, and hsa-let-7i-5p), targeting the CT genesGAGE1,SSX2,PRAME,SCP1, and the gene for DNA methyltransferase 3A (DNMT3A), were found. Data on the mechanisms of the transcriptional activity regulation of CT genes in malignant colon tumors are important for the development of CTA-dependent immunotherapeutic approaches for the treatment of this type of tumor.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据