期刊
MOLECULAR & CELLULAR PROTEOMICS
卷 19, 期 11, 页码 1739-1748出版社
ELSEVIER
DOI: 10.1074/mcp.R120.002234
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资金
- National Cancer Institute of the National Institutes of Health [R33 CA225248]
MS-based proteome profiling has become increasingly comprehensive and quantitative, yet a persistent short-coming has been the relatively large samples required to achieve an in-depth measurement. Such bulk samples, typically comprising thousands of cells or more, provide a population average and obscure important cellular heterogeneity. Single-cell proteomics capabilities have the potential to transform biomedical research and enable understanding of biological systems with a new level of granularity. Recent advances in sample processing, separations and MS instrumentation now make it possible to quantify >1000 proteins from individual mammalian cells, a level of coverage that required an input of thousands of cells just a few years ago. This review discusses important factors and parameters that should be optimized across the workflow for single-cell and other low-input measurements. It also highlights recent developments that have advanced the field and opportunities for further development.
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