期刊
MICROCHIMICA ACTA
卷 187, 期 9, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-020-04506-1
关键词
Faraday cage-type aptasensor; Dual-mode detection; Vibrio parahaemolyticus; Ru-MOF two-dimensional material; Electrochemiluminescence; Differential pulse voltammetry
资金
- National Natural Science Foundation of China [41576098, 81773483]
- State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products [ZS20190101, KF20190101]
- K.C. Wong Magna Fund at Ningbo University
A Faraday cage-type aptasensor has been developed for dual-mode detection of a common bacterial pathogenVibrio parahaemolyticus(VP) by electrochemiluminescence (ECL) and differential pulse voltammetry (DPV), using a multi-functionalized material Pb2+-Ru-MOF@Apt2 as signal unit. The recognition aptamer Apt2 recognizes VP; specifically, ruthenium-based metal organic framework (Ru-MOF) and lead ions (Pb2+) embedded produce an ECL signal at a working potential from 0 to 1.5 V and DPV signal from - 0.3 to - 0.8 V vs. Ag/AgCl. Since Ru-MOF is a two-dimensional conductive material signal unit overlapped onto the electrode surface to form a Faraday cage-type aptasensor. Thus, electrons could be easily exchanged between electrode and signal tags without being hindered by micron-size VP, resulting in a high detection sensitivity with a detection limit of 1.7 CFU mL(-1). In addition, dual-mode detection was achieved, improving the accuracy and reliability of rapid field detection. Stability and selectivity were also satisfactory. The tests of real samples indicate that this Faraday cage-type aptasensor is suited for rapid detection of
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