期刊
MICROCHEMICAL JOURNAL
卷 160, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.microc.2020.105596
关键词
microRNA-21; Impedimetric biosensor; Biomarker; Graphene oxide; Chitosan
资金
- CONICET
- ANPCyT [PICT 2016-1261]
- SECyT-UNC (Argentina)
- ECOS-SUD program [A16E02]
- Chinese Scholarship Council
An impedimetric genosensor was developed for quantifying microRNA-21, allowing label-free, nonamplified, simple, and fast biosensing in human blood serum, urine, and saliva samples.
We report here an impedimetric genosensor for the quantification of microRNA-21 using [Fe(CN)(6)](3-/4-) as redox probe to transduce the hybridization event. The biosensing platform was built at a thiolated-gold electrode by covalent bond of reduced graphene oxide (RGO) modified with chitosan (CHIT) and further covalent attachment of the aminated DNA probe. GO was used to provide the carboxylic groups for the covalent attachment of CHIT and, once reduced, to improve the electroactivity of the resulting platform, while CHIT served as a bridge between the thiol and the aminated probe DNA. The proposed bioanalytical platform allows the label-free , nonamplified, simple and fast biosensing of microRNA-21 , with a linear range between 1.0 x 10(-12) M and 1.0 x 10(-8) M, a sensitivity of (134 +/- 4) Omega M-1 (r(2) = 0.996) , a detection limit of 300 fM, and a reproducibility of 5.9% for 1.0 x 10(-12) M miRNA-21 and 2.2% for 1.0 x 10(-9) M miRNA-21. The genosensor was successfully used for the quantification of microRNA-21 in enriched human blood serum, urine and saliva samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据