A simple and efficient cyclic square wave voltammetric method for simultaneous determination of epinephrine and norepinephrine using an activated pencil graphite electrode

Simultaneous determination of catecholamine neurotransmitters epinephrine (EP) and norepinephrine (NP) using an electrochemically activated pencil graphite electrode (PGE*) is reported. Response characteristics of the PGE* toward the mentioned compounds were investigated by cyclic square wave voltammetry. In a mixture of analytes the peak currents of EP and NP increased with concentrations between 2.5 x 10(-6) and 2.5 x 10(-4) M. Detection limits of 8.32 x 10(-7) M for EP and 9.92 x 10(-7) M for NP were attained. The method was applied for their quantification in pharmaceutical and human plasma samples.

Automated summary

The simultaneous determination of catecholamine neurotransmitters epinephrine (EP) and norepinephrine (NP) using an electrochemically activated pencil graphite electrode (PGE*) was reported. Peak currents of EP and NP increased with concentrations in a mixture of analytes, with detection limits of 8.32 x 10(-7) M for EP and 9.92 x 10(-7) M for NP. The method was successfully applied for quantification in pharmaceutical and human plasma samples.