期刊
MICROCHEMICAL JOURNAL
卷 157, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.microc.2020.104946
关键词
Dasatinib; Calf thymus ds-DNA; Drug-DNA interaction; Graphite pencil electrode; Voltammetry
资金
- Ankara University Research Fund [16L0430002, 17H0430009]
The interaction of anticancer drug dasatinib (DSB) with double-stranded calf thymus DNA (ds-DNA) was investigated by using voltammetric techniques on a single-use pencil graphite electrode (PGE). Free DSB displayed a single oxidation peak in acetate buffer solution (pH 4.80). The changes in the anodic CV and DPV peak currents of DSB were investigated in the absence and presence of DNA in aqueous media. Some kinetic parameters, such as diffusion coefficient (D), heterogeneous rate constants (k(s)), and electrode surface concentration (Gamma) of free DSB and DSB-DNA complex, were calculated using CV data. The DSB-DNA complex formation constant (K) and Gibbs free energy (Delta G degrees) were determined to be 2.51x10(4) M-1 and 25.10 kJ mol(-1), respectively. The results obtained show that there is an interaction between DSB and ds-DNA, and DSB binds to DNA through intercalation interaction. The formation equilibrium constant (K) was found to compare well with similar data reported in the literature. Besides, the thermodynamic parameters (K and Delta G degrees) obtained by voltammetric measurements were compared with UV spectroscopic data and were found to be approximately of the same order (4.49x10(4) M-1).
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