期刊
MARINE DRUGS
卷 18, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/md18080430
关键词
Nibea japonica; swim bladder; marine collagen peptides; antioxidant activity
资金
- National Spark Program of China [2015GA700044]
- Science and Technology Bureau of Zhoushan [2012C23023]
Five different proteases were used to hydrolyze the swim bladders ofNibea japonicaand the hydrolysate treated by neutrase (collagen peptide named SNNHs) showed the highest DPPH radical scavenging activity. The extraction process of SNNHs was optimized by response surface methodology, and the optimal conditions were as follows: a temperature of 47.2 degrees C, a pH of 7.3 and an enzyme concentration of 1100 U/g, which resulted in the maximum DPPH clearance rate of 95.44%. Peptides with a Mw of less than 1 kDa (SNNH-1) were obtained by ultrafiltration, and exhibited good scavenging activity for hydroxyl radicals, ABTS radicals and superoxide anion radicals. Furthermore, SNNH-1 significantly promoted the proliferation of HUVECs, and the protective effect of SNNH-1 against oxidative damage of H2O2-induced HUVECs was investigated. The results indicated that all groups receiving SNNH-1 pretreatment showed an increase in GSH-Px, SOD, and CAT activities compared with the model group. In addition, SNNH-1 pretreatment reduced the levels of ROS and MDA in HUVECs with H2O2-induced oxidative damage. These results indicate that collagen peptides from swim bladders ofNibea japonicacan significantly reduce the oxidative stress damage caused by H(2)O(2)in HUVECs and provides a basis for the application of collagen peptides in the food industry, pharmaceuticals, and cosmetics.
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