Blood oxygenation level-dependent cardiovascular magnetic resonance of the skeletal muscle in healthy adults: Different paradigms for provoking signal alterations

Purpose Stress blood oxygenation level-dependent (BOLD) cardiovascular magnetic resonance allows for quantitative evaluation of blood flow reserve in skeletal muscles. This study aimed to prospectively compare three commonly used skeletal BOLD cardiovascular magnetic resonance paradigms in healthy adults: gas inhalation, cuff compression-induced ischemia and postocclusive reactive hyperemia, and exercise. Methods Twelve young (22 +/- 0.9 years) and 10 elderly (58 +/- 5.0 years) healthy subjects underwent BOLD cardiovascular magnetic resonance under the three paradigms.T2*signal intensity time curves were generated and quantitative parameters were calculated. Meanwhile, stress transcutaneous oxygen pressure measurements were obtained as comparison. Measurement reproducibility was assessed with intraclass correlation coefficients. Differences in theT2*BOLD variation, the correlation with transcutaneous oxygen pressure, and the age-related change between paradigms were statistically analyzed. Results Minimum ischemic value and maximum hyperemic peak value showed the highest interobserver and interscan reproducibilities (intraclass correlation coefficient >0.90). The plantar dorsiflexion exercise paradigm elicited the largestT2*BOLD variation (15.48% +/- 10.56%), followed by ischemia (8.30% +/- 6.33%). Negligible to weak changes were observed during gas inhalation. Correlations with transcutaneous oxygen pressure measurements were found in the ischemic phase (r= 0.966;P< .001) and in the postexercise phase (r= -0.936;P< .001). Minimum ischemic value, maximum hyperemic peak value, maximum postexercise value, and slope of postexercise signal decay showed significant differences between young and elderly subjects (P< .01). Conclusion Ischemia and reactive hyperemia have superior reproducibility, and exercise could induce the largestT2*variation. Key parameters from the two paradigms show age-related differences.

Automated summary

The study compared three commonly used skeletal BOLD cardiovascular magnetic resonance paradigms in healthy adults: gas inhalation, cuff compression-induced ischemia and reactive hyperemia, and exercise. Ischemia and reactive hyperemia were found to have superior reproducibility, while exercise induced the largest T2* variation. Key parameters from the two paradigms showed age-related differences in young and elderly subjects.