期刊
LIPIDS
卷 56, 期 1, 页码 111-122出版社
WILEY
DOI: 10.1002/lipd.12277
关键词
Fatty acid analysis; Monounsaturated fatty acids (MUFA); NMR; Polyunsaturated fatty acids (PUFA); Saturated fatty acids; Triacylglycerol analysis
资金
- Norwegian Agriculture Agency, via the Research Council of Norway as a part of the project Effects of lipids composition and structure in meat and dairy foods on digestibility and low-grade inflammation in cell, animals and humans [281207]
- Research Council of Norway through the Norwegian NMR Platform, NNP [226244/F50]
The study analyzed specific fatty acids in pig and beef tissues using high-resolution NMR, obtaining quantitative data for composition and distribution. A method for determining saturated fatty acid composition in pork was also developed. The results could support decisions related to fatty acid profiles and stimulate further method developments in high field NMR.
The uptake of specific fatty acids in humans is dependent on their position on the glycerol backbone. There is a great interest in methods that can access this information fast and accurately. By way of high-resolution NMR, we have analyzed TAG extracted from pig and beef tissues and obtained quantitative data for the composition and regioisomeric distribution of all major unsaturated fatty acids usually found in these source materials, using a combination of manual integration and deconvolution of(13)C NMR spectra. In addition, we have developed a method for determining composition and regioisomeric distribution of the two main saturated fatty acids found in pork (16:0, 18:0). The results are discussed in relation to species-specific genetic characteristics of fatty acid and TAG biosynthesis. The developed method could support decisions related to breeding for desired fatty acid profiles, and stimulate further methodology developments using high field NMR.
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