期刊
LIFE SCIENCES
卷 257, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2020.118042
关键词
Exosome; Smoke inhalation; Lung injury; HMGB1; Mesenchymal stem cell
资金
- National Natural Science Foundation of China [81760342]
- Key Program of Natural Science Foundation of Jiangxi Province of China [2017lACB20031]
- Gan Po Talent 555 Project Leading Talent Training Program of Science and Technology Department of Jiangxi Province of China [1210013001]
- Science and Technology Research Program of Education Department of Jiangxi Province of China [GTTl60019]
- Key Research and Development Program of Science and Technology Department of Jiangxi Province of China [2017lACG70004]
Aims: To investigate the role of bone marrow mesenchymal stem cell (BMSC)-derived exosomes in smoke inhalation lung injury. Main methods: In this study, we initially isolated exosomes from BMSCs and identified them by western blot and transmission electron microscopy. BMSC-derived exosomes were then used to treat in vitro and in vivo models of smoke inhalation lung injury. Pathologic alterations in lung tissue, the levels of inflammatory factors and apoptosis-related factors, and the expression of HMGB1 and NF-kappa B were determined to evaluate the therapeutic effect of BMSC-derived exosomes. Key findings: We found that BMSC-derived exosomes could alleviate the injury caused by smoke inhalation. Smoke inhalation increased the levels of inflammatory factors and apoptosis-related factors and the expression of HMGB1 and NF-kappa B, and these increases were reversed by BMSC-derived exosomes. HMGB1 overexpression abrogated the exosome-induced decreases in inflammatory factors, apoptosis-related factors and NF-kappa B. Significance: Collectively, these results indicate that BMSC-derived exosomes can effectively alleviate smoke inhalation lung injury by inhibiting the HMGB1/NF-kappa B pathway, suggesting that exosome, a noncellular therapy, is a potential therapeutic strategy for inhalation lung injury.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据