期刊
JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS
卷 44, 期 2, 页码 223-237出版社
WILEY
DOI: 10.1111/jvp.12913
关键词
antimicrobial resistance; surveillance; whole genome sequencing
资金
- Defense Advanced Research Projects Agency [HR0011937807]
- National Institute for Allergy and Infectious Diseases [75N93019C00076]
Laboratory identification of antibacterial resistance is crucial in infectious disease medicine and can be done through genetic testing and whole genome sequencing. These developments provide a new paradigm for detecting and tracking antimicrobial resistance for both clinical and public health purposes.
The laboratory identification of antibacterial resistance is a cornerstone of infectious disease medicine. In vitro antimicrobial susceptibility testing has long been based on the growth response of organisms in pure culture to a defined concentration of antimicrobial agents. By comparing individual isolates to wild-type susceptibility patterns, strains with acquired resistance can be identified. Acquired resistance can also be detected genetically. After many decades of research, the inventory of genes underlying antimicrobial resistance is well known for several pathogenic genera including zoonotic enteric organisms such asSalmonellaandCampylobacterand continues to grow substantially for others. With the decline in costs for large scale DNA sequencing, it is now practicable to characterize bacteria using whole genome sequencing, including the carriage of resistance genes in individual microorganisms and those present in complex biological samples. With genomics, we can generate comprehensive, detailed information on the bacterium, the mechanisms of antibiotic resistance, clues to its source, and the nature of mobile DNA elements by which resistance spreads. These developments point to a new paradigm for antimicrobial resistance detection and tracking for both clinical and public health purposes.
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