期刊
JOURNAL OF THROMBOSIS AND THROMBOLYSIS
卷 51, 期 4, 页码 905-914出版社
SPRINGER
DOI: 10.1007/s11239-020-02310-5
关键词
Immune thrombocytopenia; Apoptosis; PI3K-akt signalling pathway
资金
- Taishan Youth Scholar Foundation of Shandong Province [tsqn201812140]
- Academic promotion programme of Shandong First Medical University [2019RC018]
- Taishan Scholar Foundation of Shandong Province
- National Natural Science Foundation of China [81570104]
- Key Research and Development Project of Jinan [201907021, 201907026]
- Key Research and Development Program of Shandong Province [2018CXGC1213]
- Technology Development Projects of Shandong Province [2017GSF18189]
This study investigated the differences in plasma protein expression between ITP patients and healthy controls using mass spectrometry-based proteomics analysis. Differentially expressed proteins associated with apoptosis were identified, and further confirmed through PRM quantification. The findings highlighted the significant impact of apoptosis on ITP pathogenesis, potentially through the PI3K-Akt signaling pathway.
To investigate differences in the expression of plasma proteins in immune thrombocytopenia (ITP) and normal control groups, bone marrow samples were collected from 20 active ITP patients and 20 healthy controls. Quantitative proteomics analysis based on mass spectrometry was used to measure the protein levels and understand the protein networks. We found differentially expressed proteins in ITP patients and healthy controls. Parallel reaction monitoring (PRM), a targeted proteome quantification technique, was used to quantitatively confirm the identified target proteins and verify the proteomics data. In this study, a total of 829 proteins were identified, and the fold-change cut-off was set at 1.5 (patients vs controls); a total of 26 proteins were upregulated, and 69 proteins were downregulated. The bioinformatics analysis indicated that some differentially expressed proteins were associated with apoptosis. KEGG enrichment analysis showed that the apoptosis-related proteins were closely related to the PI3K-Akt signalling pathway. PRM demonstrated that apoptosis-related proteins were significantly decreased in ITP patients, which further confirmed the important effect of apoptosis on ITP pathogenesis. We hypothesised that apoptosis may be closely related to ITP pathogenesis through the PI3K-Akt signalling pathway.
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